Journal: iScience

Article Title: Adipocyte-specific Steap4 deficiency reduced thermogenesis and energy expenditure in mice

doi: 10.1016/j.isci.2025.111903

Figure Lengend Snippet: STEAP4 is highly expressed in adipose tissue and mature adipocytes, and its deficiency in adipocytes promotes HFD-induced obesity (A) Bulk tissue gene expression for STEAP4 in human tissues reported by GTEx. (B) Steap4 expression measured in selected mouse tissues by western blot. (C) Steap4 expressions analyzed in isolated SVF (stromal vascular fraction) or mature adipocytes by western blot. (D) Body weight of Steap4 f/f and Steap4 AKO mice fed an HFD diet for 12 weeks ( n = 7, 6). (E) Indicated organs weight from HFD-fed Steap4 f/f and Steap4 AKO mice after 12 weeks HFD feeding ( n = 6 pairs). (F) EchoMRI analysis of fat mass and lean mass from Steap4 f/f and Steap4 AKO mice after HFD feeding ( n = 7, 6). (G–H) Glucose tolerance test (GTT) and insulin tolerance test (ITT) in HFD-fed Steap4 f/f and Steap4 AKO mice ( n = 7, 6). (I) Upper: Representative images (20x) of Hematoxylin and Eosin (H&E) staining and Mac2 staining of iWAT sections from HFD-fed Steap4 f/f and Steap4 AKO mice. Scale bars: 150 μm. Lower: Quantification of crown-like structure (CLS) numbers in 10x objective ( n = 12 fields). (J) Left: Representative images of H&E staining and Ucp1 staining of iBAT sections from HFD-fed Steap4 f/f and Steap4 AKO mice. Right: Quantification of relative Ucp1 + area by ImageJ Software ( n = 12 fields). Scale bars: 150 μm (H&E) and 75 μm (Ucp1). (K) Expression of the indicated mRNAs in iBAT from HFD-fed Steap4 f/f and Steap4 AKO mice ( n = 6 each group). Statistical significance for all relevant graphs was determined by two-tailed Student’s test. Significance: ∗ 0.01 ≤ p < 0.05, ∗∗ 0.001 ≤ p < 0.01, ∗∗∗ p < 0.001; ns, not significant. All data represent means ± SEM.

Article Snippet: Sections on slides were further blocked with 3 % BSA in PBS solution for at least 30 min. Anti-Mac2 monoclonal antibody (Cedarlane, CL8942AP; 0.5 μg/ml) or anti-UCP1 monoclonal antibody (Abcam, ab261912, dilution 1: 50) was incubated at 4°C overnight.

Techniques: Gene Expression, Expressing, Western Blot, Isolation, Staining, Software, Two Tailed Test

Journal: iScience

Article Title: Adipocyte-specific Steap4 deficiency reduced thermogenesis and energy expenditure in mice

doi: 10.1016/j.isci.2025.111903

Figure Lengend Snippet:

Article Snippet: Sections on slides were further blocked with 3 % BSA in PBS solution for at least 30 min. Anti-Mac2 monoclonal antibody (Cedarlane, CL8942AP; 0.5 μg/ml) or anti-UCP1 monoclonal antibody (Abcam, ab261912, dilution 1: 50) was incubated at 4°C overnight.

Techniques: Recombinant, SYBR Green Assay, Real-time Polymerase Chain Reaction, Reverse Transcription, Isolation, BIA-KA, RNA Sequencing, Mass Spectrometry, Expressing, Alternative Splicing, Software, Functional Assay